Characterization of murine peritoneal macrophage receptors for fibrin(ogen) degradation products

Blood. 1986 May;67(5):1224-8.

Abstract

The binding of human fibrinogen degradation fragments D1, E, X, and Y, as well as fibrin fragment D1 dimer, to mouse peritoneal macrophages was examined. A Scatchard plot of fragment D1 binding was biphasic, suggesting two classes of receptors. Fragments D1, D1 dimer, X, and Y in low concentrations bound to macrophages with high affinity (Kd = 23 to 73 X 10(-11) mol/L). Fragment E bound specifically but at a much lower level than the other fragments. Fragment D1 was able to compete for the binding of radiolabeled fragments X and Y but not radiolabeled fragment E. These studies indicate that fragments D and E are recognized by separate receptor systems but that all of the fibrinogen degradation products that contain the D domain are recognized by the same receptor system.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Ascitic Fluid
  • Binding, Competitive
  • Chromatography, High Pressure Liquid
  • Fibrin Fibrinogen Degradation Products / metabolism*
  • Kinetics
  • Macromolecular Substances
  • Macrophages / metabolism*
  • Mice
  • Receptors, Cell Surface / metabolism*
  • Receptors, Peptide*

Substances

  • Fibrin Fibrinogen Degradation Products
  • Macromolecular Substances
  • Receptors, Cell Surface
  • Receptors, Peptide
  • fibrin-fibrinogen degradation products receptor
  • fibrinogen D fragment
  • fibrinogen fragment E
  • fibrinogen fragment X
  • fibrinogen fragment Y