Effect of Anthocyanin-Rich Tart Cherry Extract on Inflammatory Mediators and Adipokines Involved in Type 2 Diabetes in a High Fat Diet Induced Obesity Mouse Model

Nutrients. 2019 Aug 21;11(9):1966. doi: 10.3390/nu11091966.


Male C57BL/6J mice were used to determine the possible therapeutic effects of our previously described tart cherry extract in a chronic obesity mouse model on metabolic parameters, glucose tolerance, inflammatory mediators, and antioxidant capacity. The control group received standard mouse chow, and the high fat control group was switched to a high fat diet and tap water supplemented with 5% sucrose. The high fat + anthocyanin group received the high fat and sucrose diet, but received the anthocyanin-rich tart cherry extract dissolved in their drinking water. After six weeks, an oral glucose tolerance test was performed, and the water-soluble antioxidant capacity (ACW), superoxide dismutase (SOD) activity, and the plasma levels of insulin, C-peptide, leptin, IL-6, MCP-1, adiponectin and resistin were measured. The high fat diet increased body weight, reduced glucose tolerance, and caused an elevation in leptin, IL-6, MCP-1, and resistin levels. Furthermore, antioxidant capacity was decreased with a significant elevation of SOD activity. Anthocyanin treatment failed to reverse the effects of the high fat diet on body weight and glucose tolerance, but significantly reduced the leptin and IL-6 levels. The tart cherry extract also made a significant enhancement in antioxidant capacity and SOD activity. Our results show that chronic anthocyanin intake has a potential to enhance redox status and alleviate inflammation associated with obesity.

Keywords: adipokines; anthocyanins; inflammatory mediators; mouse; obesity; sour cherry; type 2 diabetes.

MeSH terms

  • Adipokines
  • Adiponectin
  • Animals
  • Anthocyanins / chemistry*
  • Antioxidants
  • Diabetes Mellitus, Type 2 / chemically induced
  • Diet, High-Fat / adverse effects*
  • Glucose Tolerance Test
  • Humans
  • Inflammation / metabolism*
  • Interleukin-6 / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / chemically induced*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Prunus avium / chemistry*
  • Resistin
  • Superoxide Dismutase


  • Adipokines
  • Adiponectin
  • Anthocyanins
  • Antioxidants
  • Interleukin-6
  • Plant Extracts
  • Resistin
  • interleukin-6, mouse
  • Superoxide Dismutase