Effect of sample preparation and liquid chromatography column choice on selectivity and precision of plasma catecholamine determination

J Chromatogr. 1987 Jan 16:386:19-24. doi: 10.1016/s0021-9673(01)94580-7.


Research into the role of the catecholamines has often depended on the reliable determination of plasma catecholamine concentrations, which present a challenge since they are normally in the low pg/ml range. Most methods employ liquid chromatography, with variations in sample preparation, the separation mechanism, and detection. We tested a new approach to sample clean-up using boric acid gel instead of alumina. No advantage was found. We also compared cation-exchange separation with ion-pair chromatography. Several improvements are possible with the former, most notably greater precision, better specificity, and increased throughput.

MeSH terms

  • Aluminum Oxide
  • Catecholamines / blood*
  • Chromatography, Liquid
  • Humans
  • Indicators and Reagents
  • Quality Control


  • Catecholamines
  • Indicators and Reagents
  • Aluminum Oxide