Dectin-1 as a Potential Inflammatory Biomarker for Metabolic Inflammation in Adipose Tissue of Individuals with Obesity

Cells. 2022 Sep 15;11(18):2879. doi: 10.3390/cells11182879.


In obesity, macrophage activation and infiltration in adipose tissue (AT) underlie chronic low-grade inflammation-induced insulin resistance. Although dectin-1 is primarily a pathogen recognition receptor and innate immune response modulator, its role in metabolic syndromes remains to be clarified. This study aimed to investigate the dectin-1 gene expression in subcutaneous AT in the context of obesity and associated inflammatory markers. Subcutaneous AT biopsies were collected from 59 nondiabetic (lean/overweight/obese) individuals. AT gene expression levels of dectin-1 and inflammatory markers were determined via real-time reverse transcriptase-quantitative polymerase chain reaction. Dectin-1 protein expression was assessed using immunohistochemistry. Plasma lipid profiles were measured by ELISA. AT dectin-1 transcripts and proteins were significantly elevated in obese as compared to lean individuals. AT dectin-1 transcripts correlated positively with body mass index and fat percentage (r ≥ 0.340, p ≤ 0.017). AT dectin-1 RNA levels correlated positively with clinical parameters, including plasma C-reactive protein and CCL5/RANTES, but negatively with that of adiponectin. The expression of dectin-1 transcripts was associated with that of various proinflammatory cytokines, chemokines, and their cognate receptors (r ≥ 0.300, p ≤ 0.05), but not with anti-inflammatory markers. Dectin-1 and members of the TLR signaling cascade were found to be significantly associated, suggesting an interplay between the two pathways. Dectin-1 expression was correlated with monocyte/macrophage markers, including CD16, CD68, CD86, and CD163, suggesting its monocytes/macrophage association in an adipose inflammatory microenvironment. Dectin-1 expression was independently predicted by CCR5, CCL20, TLR2, and MyD88. In conclusion, dectin-1 may be regarded as an AT biomarker of metabolic inflammation in obesity.

Keywords: adipose tissue; dectin-1; metabolic inflammation; obesity; proinflammatory markers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin* / metabolism
  • Adipose Tissue / metabolism
  • Biomarkers / metabolism
  • C-Reactive Protein / metabolism
  • Chemokine CCL5* / metabolism
  • Cytokines / metabolism
  • Humans
  • Inflammation / pathology
  • Lectins, C-Type* / metabolism
  • Lipids
  • Myeloid Differentiation Factor 88 / metabolism
  • Obesity / metabolism
  • RNA / metabolism
  • RNA-Directed DNA Polymerase / metabolism
  • Toll-Like Receptor 2 / metabolism


  • Adiponectin
  • Biomarkers
  • CLEC7A protein, human
  • Chemokine CCL5
  • Cytokines
  • Lectins, C-Type
  • Lipids
  • Myeloid Differentiation Factor 88
  • Toll-Like Receptor 2
  • RNA
  • C-Reactive Protein
  • RNA-Directed DNA Polymerase

Grants and funding

This study was funded by Kuwait Foundation for Advancement of Sciences (KFAS) (Grant #: RA2010-003, June 2010).