Inhibition of EV71 replication by an interferon-stimulated gene product L3HYPDH

Virus Res. 2024 Apr:342:199336. doi: 10.1016/j.virusres.2024.199336. Epub 2024 Feb 13.

Abstract

Enterovirus 71 (EV71) is the common causative agent of hand-foot-mouth disease (HFMD). Despite evidence in mice model suggested that the interferon (IFN) signaling pathways play a role in defending against this virus, knowledge on the IFN-mediated antiviral response is still limited. Here we identified an IFN-stimulated gene (ISG) called L3HYPDH, whose expression inhibits EV71 replication. Mapping assay indicated that amino acids 61-120 and 295-354 are critical for its optimal antiviral activity. Mechanismly, L3HYPDH specifically inhibits protein translation mediated by EV71 internal ribosome entry site (IRES). Our data thus uncovered a new mechanism utilized by the host cell to restrict EV71 replication.

Keywords: Antiviral activity; Enterovirus 71; Interferon-stimulated gene; L3HYPDH.

MeSH terms

  • Amino Acids
  • Animals
  • Antiviral Agents
  • Hand, Foot and Mouth Disease*
  • Interferons*
  • Mice
  • RNA

Substances

  • Interferons
  • RNA
  • Amino Acids
  • Antiviral Agents