Folic acid compounds in romaine lettuce

Can J Biochem. 1977 Aug;55(8):865-8. doi: 10.1139/o77-127.


The composition of folate coenzymes in romaine lettuce was studied. Lettuce extract was purified on QAE-Sephadex A-25 and folate compounds were separated into a monoglutamate fraction and a polyglutamate fraction by chromatography on Sephadex G-15. Both the mono- and poly-glutamate fractions were resolved on DEAE-cellulose. Positive identification of DEAE peaks was made by further cochromatography with high specific activity radioactive marker folate compounds and with differential microbiological assay. The distribution of folate compounds in lettuce is as follows: 32% 5-CH3-H4PteGlu; 1% 5-CHO-H4PteGlu; 3% 5-CHO-H4PteGlu4; 9% 5-CH3-H4PteGlu4; 13% 5-CHO-H4PteGlu5; and 31% 5-CH3-H4PteGlu5.

MeSH terms

  • Biological Assay
  • Enterococcus faecalis
  • Folic Acid / analysis*
  • Lacticaseibacillus casei
  • Plants / analysis*


  • Folic Acid