Dechlorination of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane by Aerobacter aerogenes. I. Metabolic products

Appl Microbiol. 1967 May;15(3):569-74. doi: 10.1128/am.15.3.569-574.1967.


Whole cells or cell-free extracts of Aerobacter aerogenes catalyze the degradation of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) in vitro to at least seven metabolites: 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE); 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (DDD); 1-chloro-2,2-bis(p-chlorophenyl)ethylene (DDMU); 1-chloro-2,2-bis(p-chlorophenyl)ethane (DDMS); unsym-bis(p-chlorophenyl)ethylene (DDNU); 2,2-bis(p-chlorophenyl)acetate (DDA); and 4,4'-dichlorobenzophenone (DBP). The use of metabolic inhibitors together with pH and temperature studies indicated that discrete enzymes are involved. By use of the technique of sequential analysis, the metabolic pathway was shown to be: DDT --> DDD -->DDMU -->DDMS --> DDNU --> DDA --> DBP, or DDT --> DDE. Dechlorination was marginally enhanced by light-activated flavin mononucleotide.

MeSH terms

  • Cyanides / pharmacology
  • DDT / metabolism*
  • Enterobacter / drug effects
  • Enterobacter / metabolism*
  • Fluorides / pharmacology
  • Iodoacetates / pharmacology
  • Malonates / pharmacology


  • Cyanides
  • Iodoacetates
  • Malonates
  • DDT
  • Fluorides