On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2

J Exp Med. 1983 Apr 1;157(4):1077-88. doi: 10.1084/jem.157.4.1077.


Generation of anti-sheep erythrocyte plaque-forming cells (PFC) is greatly reduced in the presence of monoclonal Ly-5 alloantibody. Although Ly-5 is expressed in one of its molecular forms on T cells and macrophages (M phi ) involved in this response, the only demonstrated action of Ly-5 antibody was on B cells. Evidence from elimination of Lyt-2+ cells, and from the responses of serial proportions of Ly-5.1 and Ly-5.2 cells and of Ly-5 heterozygous cells, signifies that PFC reduction cannot be ascribed to any known mechanism of suppression or to a direct suppressive action of Ly-5 antibody on B cells. A critical distinction of Ly-5 from Lyb-2 is that Ly-5 antibody reduces PFC generation to trinitrophenylated Ficoll, a thymus-independent type 2 antigen requiring T cells and M phi for maximal PFC generation in vitro. A second distinction is that PFC reduction by Ly-5 antibody is strictly tied to the time of operation of M phi factor, whereas PFC reduction by Lyb-2 antibody relates to the time of B cell triggering by antigen. Accordingly, M phi factor competitively and quantitatively inhibits the action of Ly-5 antibody in reducing PFC generation. It is likely that the Ly-5 system is concerned in the reception or handling of M phi message by B cells.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody-Producing Cells / cytology
  • Antibody-Producing Cells / immunology
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Surface / classification
  • Antigens, Surface / genetics
  • Antigens, Surface / immunology*
  • Antigens, T-Independent / immunology
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Cell Differentiation
  • Hemolytic Plaque Technique
  • Immune Tolerance
  • Isoantibodies / physiology
  • Kinetics
  • Lymphocyte Activation*
  • Lymphocyte Culture Test, Mixed
  • Macrophages / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • T-Lymphocytes / immunology


  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Surface
  • Antigens, T-Independent
  • Isoantibodies