Sequences encoding eight tRNAs and two stable RNAs of bacteriophage T4 are grouped together on the T4 genome in two clusters, separated by approximately 500 base pairs. The DNA sequence of part of this region was determined. Within each cluster coding sequences are separated by only one or a few base pairs. These findings imply that the RNAs may be processed from a single multimeric transcript, with initial endonucleolytic cleavages generating the previously characterized monomeric and dimeric precursors.