Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells

J Immunol. 1984 Jul;133(1):157-65.


The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acute Disease
  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Antigen-Antibody Reactions
  • Antigens, Surface / immunology*
  • Antigens, Surface / isolation & purification
  • Bone Marrow / immunology
  • Bone Marrow Cells
  • Cell Line
  • Cell Separation
  • Chemical Precipitation
  • Female
  • Flow Cytometry
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / immunology*
  • Hematopoietic Stem Cells / pathology
  • Humans
  • Leukemia / immunology
  • Leukemia / pathology
  • Leukemia, Erythroblastic, Acute / immunology*
  • Mice
  • Mice, Inbred BALB C


  • Antibodies, Monoclonal
  • Antigens, Surface