Modification of the fungi-fluor and the genetic systems fluorescent antibody methods for detection of Pneumocystis carinii in bronchoalveolar lavage specimens

Arch Pathol Lab Med. 1995 Feb;119(2):142-7.


Four hundred forty-five bronchoalveolar lavage specimens from patients with the human immunodeficiency virus were preserved in Saccomano's fixative and stained for Pneumocystis carinii cysts by a modified method with Fungi-Fluor Solution A (Polysciences, Warrington, Pa) and the Genetic Systems Pneumocystis carinii Immunofluorescence Antibody (Genetic Systems Corp, Seattle, Wash). The majority of patients had been treated for suspected P carinii pneumonia for a few days prior to collection of bronchoalveolar lavage specimens. P carinii cysts were detected in 194 (43.6%) specimens. Both stains identified P carinii in 166 (37.3%) specimens and were negative in 251 (56.4%), yielding a concordance rate of 93.7%. P carinii cysts were detected in 25 (5.6%) specimens by the Genetic Systems stain only, and in 3 (0.7%) specimens by the Fungi-Fluor stain only. The sensitivity for detecting cysts of P carinii was significantly greater with the Genetic Systems stain (P < .01).

Publication types

  • Comparative Study

MeSH terms

  • Bronchoalveolar Lavage Fluid / microbiology*
  • Fluorescent Antibody Technique*
  • HIV Infections / complications
  • HIV Infections / microbiology*
  • Humans
  • Pneumocystis / isolation & purification*
  • Pneumonia, Pneumocystis / complications
  • Pneumonia, Pneumocystis / microbiology*
  • Sensitivity and Specificity
  • Staining and Labeling
  • Tissue Fixation / methods