BTS1 encodes a geranylgeranyl diphosphate synthase in Saccharomyces cerevisiae

J Biol Chem. 1995 Sep 15;270(37):21793-9. doi: 10.1074/jbc.270.37.21793.


Protein prenylation utilizes different types of isoprenoids groups, namely farnesyl and geranylgeranyl, to modify proteins. These lipophilic moieties attach to carboxyl-terminal cysteine residues to promote the association of soluble proteins to membranes. Most prenylated proteins are geranylgeranylated. Geranylgeranylation is catalyzed by two different prenyltransferases, the type I and type II geranylgeranyl transferases, both of which utilize geranylgeranyl diphosphate as a lipid donor. In the yeast Saccharomyces cerevisiae, the BET2 gene encodes the beta-subunit of the type II geranylgeranyl transferase. Mutations in this gene cause a defect in the geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic. In an attempt to analyze those genes whose products may interact with Bet2, we isolated a suppressor of the bet2-1 mutant. This suppressor gene, called BTS1, encodes the yeast geranylgeranyl diphosphate synthase. BTS1 is not essential for the vegetative growth of cells; however, disrupting it impedes the geranylgeranylation of many cellular proteins and renders cells cold sensitive for growth. Our findings imply that BTS1 suppresses the bet2-1 mutant by increasing the intracellular pool of geranylgeranyl diphosphate.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkyl and Aryl Transferases*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Conserved Sequence
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Escherichia coli
  • Gene Expression
  • Genes, Fungal*
  • Genomic Library
  • Geranylgeranyl-Diphosphate Geranylgeranyltransferase
  • Kinetics
  • Molecular Sequence Data
  • Mutagenesis
  • Oxidoreductases / biosynthesis*
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Sequence Homology, Amino Acid
  • Suppression, Genetic


  • DNA, Fungal
  • Recombinant Proteins
  • Oxidoreductases
  • Alkyl and Aryl Transferases
  • Geranylgeranyl-Diphosphate Geranylgeranyltransferase

Associated data

  • GENBANK/U31632