Sequence analysis of cDNA for the VP6 protein of group A avian rotavirus: a comparison with group A mammalian rotaviruses

Arch Virol. 1995;140(3):605-12. doi: 10.1007/BF01718436.


cDNA corresponding to the genomic segment 6 of avian rotavirus strain PO-13, which has group A common and subgroup I antigens, but does not hybridize in Northern blots with RNA probes from group A mammalian rotaviruses, was cloned and sequenced. When the deduced amino acid sequence was compared between strain PO-13 and eight group A mammalian rotaviruses, the extent of homology ranged from 73-75%. An alignment of the amino acid sequences allowed us to identify three amino acids (Positions 120, 317 and 350) that may contribute to determining the subgroup epitopes. A phylogenetic tree constructed on the basis of nucleotide substitutions in the VP6 gene of nine rotaviruses strongly suggests that the avian rotavirus is an ancestral prototype of mammalian rotaviruses.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Viral*
  • Base Sequence
  • Capsid / chemistry
  • Capsid / genetics*
  • Capsid Proteins*
  • Chickens / virology*
  • DNA, Complementary / chemistry*
  • Molecular Sequence Data
  • Rotavirus / genetics*


  • Antigens, Viral
  • Capsid Proteins
  • DNA, Complementary
  • VP6 protein, Rotavirus

Associated data

  • GENBANK/D16329