Molecular cloning, sequencing and expression of a cDNA encoding a human liver NAD-dependent alpha-glycerol-3-phosphate dehydrogenase

Biochim Biophys Acta. 1995 May 17;1262(1):91-4. doi: 10.1016/0167-4781(95)00069-s.


This work reports the primary nucleotide structure and in vitro translation of a cDNA, expressed by a gene mapping on chromosome 12, that encodes a human hepatic alpha-glycerol-3-phosphate dehydrogenase (L-glycerol-3-phosphate:NAD oxidoreductase, E.C. The 1413 bp cDNA comprises an ORF of 1050 bp that encodes a 349 amino acid protein of 37.5 kDa. Northern blot analysis of poly(A)+ mRNA from human liver showed three transcripts, while from human placenta only two transcripts were detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Glycerol-3-Phosphate Dehydrogenase (NAD+)
  • Glycerolphosphate Dehydrogenase / chemistry
  • Glycerolphosphate Dehydrogenase / genetics*
  • Glycerolphosphate Dehydrogenase / metabolism
  • Humans
  • Kinetics
  • Liver / enzymology*
  • Molecular Sequence Data


  • DNA, Complementary
  • Glycerolphosphate Dehydrogenase
  • Glycerol-3-Phosphate Dehydrogenase (NAD+)

Associated data

  • GENBANK/L34041