Using protein synthesis inhibitors to establish the phylogenetic relationships of the Sulfolobales order

J Mol Evol. 1994 Nov;39(5):528-32. doi: 10.1007/BF00173422.


The sensitivity of the cell-free protein synthesis systems from Acidanus brierleyi, Acidianus infernus, and Metallosphaera sedula, members of the archaeal order Sulfolobales, to 40 antibiotics with different specificities has been studied. The sensitivity patterns were compared to those of Sulfolobus solfataricus and other archaeal, bacterial, and eukaryotic systems. The comparative analysis shows that ribosomes from the sulfolobales are the most refractory to inhibitors of protein synthesis described so far. The sensitivity results have been used to ascertain in phylogenetic relationships among the members of the order Sulfolobales. The evolutionary significance of these results are analyzed in the context of the phylogenetic position of this group of extreme thermophilic microorganisms.

Publication types

  • Comparative Study

MeSH terms

  • Anti-Bacterial Agents / classification
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / biosynthesis
  • Cell-Free System
  • Dose-Response Relationship, Drug
  • Peptide Biosynthesis
  • Peptides*
  • Phylogeny
  • Poly U / metabolism
  • Protein Biosynthesis / drug effects*
  • Protein Synthesis Inhibitors / classification
  • Protein Synthesis Inhibitors / pharmacology*
  • Sulfolobales / classification*
  • Sulfolobales / drug effects*


  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Peptides
  • Protein Synthesis Inhibitors
  • Poly U
  • polyphenylalanine