Effects of dietary protein conditioning on gentamicin-induced nephrotoxicosis in healthy male dogs

Am J Vet Res. 1994 Jan;55(1):90-7.


Eighteen 6-month-old male Beagles with normal renal function were allotted at random to 3 groups of 6 dogs each. For 21 days, each group was fed a diet that was similar except for protein content (high protein, 27.3%; medium protein, 13.7%; and low protein, 9.4%). After the conditioning period, gentamicin was administered at a dosage of 10 mg/kg of body weight, IM, every 8 hours for 8 days, and the respective diet was continued. Clearance of endogenous creatinine, 24-hour urinary excretion of protein and enzymes (gamma-glutamyltransferase, and N-acetyl-beta-D-glucosaminidase, and fractional clearance of sodium and potassium (%) were determined before and after dietary protein conditioning and on days 2, 4, 6, and 8 of gentamicin administration. Additionally, trough serum gentamicin concentration was determined on days 2, 4, 6, and 8 of gentamicin administration. At the end of the study, all dogs were euthanatized; renal histologic features were graded, using a continuous ranking scale, and renal cortical gentamicin concentrations were measured. Data were ranked and analyzed, using a nonparametric equivalent of a two-way ANOVA; P < 0.05 was considered significant. After the dietary conditioning period (prior to gentamicin), dogs fed the high-protein diet had higher endogenous creatinine clearance and urinary excretion of protein, compared with dogs fed the low-protein diet. Differences existed among groups after 8 days of gentamicin administration.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosaminidase / urine
  • Animals
  • Creatinine / metabolism
  • Dietary Proteins
  • Dogs
  • Gentamicins / toxicity*
  • Kidney / drug effects
  • Kidney / pathology*
  • Kidney / physiology
  • Kidney Cortex / drug effects
  • Kidney Cortex / pathology
  • Kidney Diseases / chemically induced
  • Kidney Diseases / pathology
  • Kidney Diseases / prevention & control*
  • Male
  • Potassium / urine
  • Random Allocation
  • Sodium / urine
  • gamma-Glutamyltransferase / urine


  • Dietary Proteins
  • Gentamicins
  • Sodium
  • Creatinine
  • gamma-Glutamyltransferase
  • Acetylglucosaminidase
  • Potassium