Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus

Nucleic Acids Res. 1994 Jan 25;22(2):208-13. doi: 10.1093/nar/22.2.208.


The DNA polymerase gene of African swine fever virus (ASFV) was mapped by marker rescue experiments using a phosphonoacetic acid-resistant mutant and hybridization with an oligonucleotide probe designed from the most conserved motif of family B DNA polymerases. Viral DNA fragments mapping in this region were cloned and sequenced. An open reading frame coding for a 1244 amino acid long peptide with a molecular mass of 142.5 kDa was determined from the sequence. A unique feature of ASFV DNA polymerase is the presence of 13 tandem repeats of the sequence Ala-Gly-Asp-Pro near the carboxyl end of the molecule. Comparison with 30 sequences of alpha-like DNA polymerases of cellular and viral origin showed that ASFV DNA polymerase has all the conserved motifs of family B DNA polymerases. A 3.9 kb transcript was detected by Northern hybridization and the transcription initiation and termination sites were mapped by S1 analysis and primer extension. Late transcription was initiated at a site different from the early transcription initiation site. A 145 kDa protein, consistent with the size of the gene, was identified by an in situ enzyme assay after gel electrophoresis of infected cell extracts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Swine Fever Virus / enzymology
  • African Swine Fever Virus / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chlorocebus aethiops
  • Conserved Sequence
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / genetics*
  • Molecular Sequence Data
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Vero Cells


  • DNA-Directed DNA Polymerase

Associated data

  • GENBANK/U27573
  • GENBANK/U27574
  • GENBANK/U27575
  • GENBANK/X73330