Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression

Mol Endocrinol. 1993 Jul;7(7):852-60. doi: 10.1210/mend.7.7.8413309.


The cytochrome P450 steroid hydroxylases are coordinately regulated by steroidogenic factor 1 (SF-1), a protein expressed selectively in steroidogenic cells. Based on its expression in steroidogenic tissues and DNA-binding specificity, we isolated a putative SF-1 cDNA from an adrenocortical cDNA library. As evidence that this cDNA encodes SF-1, we now show that it is selectively expressed in steroidogenic cells, that an antiserum against its protein product specifically abolishes the SF-1-related gel-shift complex, and that its coexpression increases promoter activity of the 21-hydroxylase 5'-flanking region in transfection experiments. Sequence analyses of the SF-1 cDNA revealed that it is the mouse homolog of fushi tarazu factor I (FTZ-F1), a nuclear receptor that regulates the fushi tarazu homeobox gene in Drosophila. A second FTZ-F1 homolog, embryonal long terminal repeat-binding protein (ELP), was recently isolated from embryonal carcinoma cells. SF-1 and ELP cDNAs are virtually identical for 1017 base pairs, including putative DNA-binding domains, but diverge at their 5'- and 3'-ends. One genomic clone contained both SF-1- and ELP-specific sequences, confirming their origin from a single gene. Characterization of this gene defined shared exons encoding common regions and alternative promoters and 3'-exons leading to differences between the two FTZ-F1 transcripts. We used in situ hybridization with transcript-specific probes to study the ontogeny of SF-1 and ELP expression. ELP transcripts were not detected from embryonic day 8 to adult, consistent with its previous isolation from embryonal carcinoma cells and its postulated role in early embryonic development.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex Neoplasms / genetics
  • Adrenal Cortex Neoplasms / pathology
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / analysis
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology
  • DNA, Neoplasm / analysis
  • DNA, Neoplasm / genetics
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Exons
  • Fushi Tarazu Transcription Factors
  • Gene Expression Regulation, Enzymologic / genetics*
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Regulator / genetics
  • Genes, Regulator / physiology
  • Homeodomain Proteins
  • In Situ Hybridization
  • Mice
  • Molecular Sequence Data
  • Neoplasms, Experimental / genetics
  • Neoplasms, Experimental / pathology
  • Promoter Regions, Genetic / genetics
  • Receptors, Cytoplasmic and Nuclear / analysis
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Cytoplasmic and Nuclear / physiology
  • Repressor Proteins / analysis
  • Repressor Proteins / genetics
  • Repressor Proteins / physiology
  • Steroid 21-Hydroxylase / genetics
  • Steroid Hydroxylases / genetics*
  • Steroidogenic Factor 1
  • Transcription Factors / analysis
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Transcription, Genetic / genetics
  • Tumor Cells, Cultured


  • Carrier Proteins
  • DNA, Neoplasm
  • DNA-Binding Proteins
  • Fushi Tarazu Transcription Factors
  • Homeodomain Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Repressor Proteins
  • Steroidogenic Factor 1
  • Transcription Factors
  • steroidogenic factor 1, mouse
  • Steroid Hydroxylases
  • Steroid 21-Hydroxylase