The polar lipids of group B Streptococci. I. Glucosylated diphosphatidylglycerol, a novel glycopholipid

Biochim Biophys Acta. 1977 Apr 26;487(1):74-88. doi: 10.1016/0005-2760(77)90045-5.


1. From group B Streptococci a novel glycophospholipid was isolated, which contained D-glucose, glycerol, acyl groups and phosphorus in a molar ratio of approx. 1:3:4:2. It was established to be 2'-O-alpha-D-glucopyranosyl-, 1',3'-bis-(1,2-diacyl-sn-glycero-3-phospho)-glycerol. 2. The structure of the deacylated core was accomplished by analyses of the breakdown products obtained on (i) strong alkaline hydrolysis, (ii) Smith-degradation, and (iii) periodate oxidation with subsequent hydrazinolysis. The four acyl groups were located by sequential degradation of the native lipid with phospholipase A2 and 98% acetic acid. 2. In group B Streptococci approximately 25% of diphosphatidylglycerol occurs in the form of its glucosylated derivative which accounts for 18% of the lipid phosphorus. The glucosylated phosphatidylglycerol analogue could not be detected. A phosphoglucolipid, however, was present, which was tentatively identified as glycerophosphodiglucosyldiacylglycerol.

MeSH terms

  • Carbohydrates / analysis
  • Cardiolipins / analysis*
  • Chromatography, DEAE-Cellulose
  • Glucose / analysis
  • Glycolipids / analysis
  • Streptococcus / analysis*


  • Carbohydrates
  • Cardiolipins
  • Glycolipids
  • Glucose