Characterization of murine intercellular adhesion molecule-2

J Immunol. 1996 Jun 15;156(12):4909-14.


Rat mAbs were raised against murine intercellular adhesion molecule-2 (ICAM-2). Immune precipitation and purification reveal that the murine ICAM-2 glycoprotein is 55 kDa and is similar in size to human ICAM-2. ICAM-2 is expressed on a variety of leukocyte cell lines, including T and B lymphoma, mastocytoma, and macrophage lines. ICAM-2 is well expressed on endothelioma cell lines, and in contrast to ICAM-1, expression is not increased by inflammatory cytokines. One of the mAb to ICAM-2 partially or completely inhibits binding of cells expressing LFA-1 to purified ICAM-2, and binding of cells expressing ICAM-2 to purified LFA-1. The findings in the mouse are congruent with those in the human, suggesting functional conservation of ICAM-2 across species.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, CD / chemistry*
  • Antigens, CD / immunology
  • Cell Adhesion
  • Cell Adhesion Molecules / chemistry*
  • Cell Adhesion Molecules / immunology
  • Cell Line
  • Cloning, Molecular
  • Humans
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Mice
  • Protein Binding


  • Antibodies, Monoclonal
  • Antigens, CD
  • Cell Adhesion Molecules
  • ICAM2 protein, human
  • Lymphocyte Function-Associated Antigen-1