Primary urine production in insect Malpighian tubules is stimulated by two classes of neuropeptides, CRF-related diuretic peptides and insect kinins. The CRF-related peptide of the locust, Locusta migratoria, has a hormonal role in the control of postfeeding diuresis, but the functional role of the kinins has yet to be defined. The two classes of peptide act synergistically to stimulate tubule secretion, and the kinins may therefore have a modulatory action in the control of diuresis. The peptides differ in their effects on Malpighian tubule ion transport, and this could be important for the regulation of hemolymph volume and composition.