Prevalence of estrogen receptor variant messenger RNAs in human breast cancer

Cancer Res. 1996 Oct 1;56(19):4324-7.


A new approach, based on the competitive amplification of wild-type and exon-deleted estrogen receptor (ER) variant cDNAs, was used to screen 100 human breast tumors for the presence of ER variants. Already described exon 4-deleted ER mRNA was preferentially detected in tumors with lower grades (P < 0.05) or higher progesterone receptor levels (P < 0.01), whereas new ER variants, deleted in exons 2-4 or in regions within exons 3-7 were associated with higher grades (P < 0.025) and higher ERs (P < 0.001). This approach allows investigation of the expression of multiple ER variant mRNAs and may implicate them as new prognostic markers and as possible contributors to tumor progression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Exons / genetics*
  • Female
  • Humans
  • Mutation
  • Pilot Projects
  • Polymerase Chain Reaction
  • Prevalence
  • RNA, Messenger / analysis*
  • RNA, Neoplasm / analysis*
  • Receptors, Estrogen / genetics*
  • Receptors, Progesterone / genetics
  • Sequence Deletion


  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Estrogen
  • Receptors, Progesterone