Abstract
Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsaturated bond of 2-cyclohexen-1-one.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Cyclohexanones / metabolism
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Enterobacter cloacae / enzymology
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Enterobacter cloacae / genetics*
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Kinetics
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Models, Chemical
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Molecular Sequence Data
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NADP / metabolism
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Nitroglycerin / metabolism
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Oxidation-Reduction
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Oxidoreductases / antagonists & inhibitors
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Oxidoreductases / genetics*
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Oxidoreductases / metabolism*
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Recombinant Proteins / biosynthesis
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Testosterone / pharmacology
Substances
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Cyclohexanones
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Recombinant Proteins
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Testosterone
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2-cyclohexen-1-one
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NADP
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Oxidoreductases
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pentaerythritol tetranitrate reductase
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Nitroglycerin