Human papillomavirus DNA in non-melanoma skin cancers of a renal transplant recipient: detection of a new sequence related to epidermodysplasia verruciformis associated types

J Invest Dermatol. 1997 Jan;108(1):53-6. doi: 10.1111/1523-1747.ep12285630.


The detection of human papillomavirus (HPV) types originally isolated from patients with epidermodysplasia verruciformis (EV) in skin tumors of transplant recipients may point to a role of this HPV subgroup in non-melanoma skin cancer in immunosuppressed people. We analyzed 17 formalin-fixed, paraffin-embedded biopsies of benign or malignant skin tumors of a renal transplant patient with unusually widespread cutaneous carcinomas. Using a nested polymerase chain reaction (PCR), HPV-specific DNA was demonstrated in 11 specimens (65%). Analysis of nine PCR amplification products revealed four different sequences related to EV-associated HPVs. Three sequences occurred only in one lesion. In six samples identical sequences were found that differed from all HPV sequences published to date and may therefore represent a novel EV-HPV type, preliminarily labeled RTRX7. RTRX7 was found in benign, premalignant, and malignant skin lesions. Alignments identified HPV12 as the closest relative of RTRX7, both in the DNA (81% homology) and in the amino acid sequence (84% homology).

MeSH terms

  • Adult
  • DNA, Viral / analysis*
  • Epidermodysplasia Verruciformis / pathology
  • Epidermodysplasia Verruciformis / virology
  • Humans
  • Kidney Transplantation*
  • Male
  • Melanoma / chemistry*
  • Papillomaviridae / genetics*
  • Polymerase Chain Reaction
  • Sequence Analysis
  • Skin Neoplasms / chemistry*
  • Skin Neoplasms / pathology


  • DNA, Viral

Associated data

  • GENBANK/U85660