The human transcription enhancer factor-1, TEF-1, can substitute for Drosophila scalloped during wingblade development

J Biol Chem. 1997 Apr 18;272(16):10664-8. doi: 10.1074/jbc.272.16.10664.


The human transcription enhancer factor-1 (TEF-1) belongs to a family of evolutionarily conserved proteins that have a DNA binding TEA domain. TEF-1 shares a 98% homology with Drosophila scalloped (sd) in the DNA binding domain and a 50% similarity in the activation domain. We have expressed human TEF-1 in Drosophila under the hsp-70 promoter and find that it can substitute for Sd function. The transformants rescue the wingblade defects as well as the lethality of loss-of-function alleles. Observation of reporter activity in the imaginal wing discs of the enhancer-trap alleles suggests that TEF-1 is capable of promoting sd gene regulation. The functional capability of the TEF-1 product was assessed by comparing the extent of rescue by heat shock (hs)-TEF-1 with that of hs-sd. The finding that TEF-1 can function in vivo during wingblade development offers a potent genetic system for the analysis of its function and in the identification of the molecular partners of TEF-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Biological Evolution
  • Conserved Sequence
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Drosophila / genetics
  • Drosophila / physiology*
  • Drosophila Proteins*
  • Gene Expression Regulation, Developmental
  • HSP70 Heat-Shock Proteins / genetics
  • Humans
  • Insect Hormones / metabolism
  • Nuclear Proteins / metabolism
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins / biosynthesis
  • Sequence Homology, Amino Acid
  • TEA Domain Transcription Factors
  • Transcription Factors / biosynthesis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Wings, Animal / growth & development*


  • DNA-Binding Proteins
  • Drosophila Proteins
  • HSP70 Heat-Shock Proteins
  • Insect Hormones
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • TEA Domain Transcription Factors
  • TEAD1 protein, human
  • Transcription Factors
  • sd protein, Drosophila