Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR, a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin

FEBS Lett. 1997 Aug 18;413(2):349-53. doi: 10.1016/s0014-5793(97)00934-4.


A group of cDNAs isolated from a subtractive ciliary body library of a normal human eye donor revealed 100% identity with TIGR a candidate gene responsible for juvenile open zangle glaucoma [Science 275 (1997) 668-670]. Several structural features of the deduced human protein have been noted: a cleavable N-terminal signal peptide, a periodic repetition at the N-terminus of leucine and arginine residues at every seventh and eleven position respectively in helix conformation (leucine zipper-like motif) exhibiting homology with myosin, and with olfactomedin in the C-terminus. The mRNA for TIGR is abundantly expressed in the ciliary body, iris, heart and skeletal muscle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Ciliary Body / chemistry
  • Ciliary Body / physiology*
  • Cloning, Molecular
  • Cytoskeletal Proteins
  • DNA, Complementary / genetics*
  • Extracellular Matrix Proteins / genetics
  • Eye / chemistry
  • Eye Proteins / genetics*
  • Gene Library
  • Glaucoma, Open-Angle / genetics*
  • Glycoproteins / genetics
  • Humans
  • Molecular Sequence Data
  • Myosins / genetics
  • Organ Specificity
  • RNA, Messenger / analysis
  • Sequence Homology, Amino Acid*
  • Sequence Homology, Nucleic Acid


  • Cytoskeletal Proteins
  • DNA, Complementary
  • Extracellular Matrix Proteins
  • Eye Proteins
  • Glycoproteins
  • RNA, Messenger
  • olfactomedin
  • trabecular meshwork-induced glucocorticoid response protein
  • Myosins

Associated data

  • GENBANK/AF001620