Expression of fibroblast growth factor 2 mRNA in early and advanced gastric cancer

Acta Oncol. 1997;36(7):695-700. doi: 10.3109/02841869709001339.

Abstract

We examined the expression of FGF-2 mRNA in 16 early and 14 advanced gastric cancer by in situ hybridisation to elucidate its role in cancer progression. Anti-sense RNA probes were synthesized by transcribing the subcloned vector with T7 RNA polymerase in the presence of digoxigenin-labeled UTP. FGF-2 mRNA was located mainly in the cytoplasm around the nuclei of endothelial cells, fibroblasts and carcinoma cells. The expression was more frequently in the diffuse type carcinomas (4/7, 57%) than in the intestinal type tumours (5/23, 22%). The survival rates of advanced gastric cancers with FGF-2 mRNA expression were significantly lower than those without FGF-2 mRNA expression (p < 0.01). No significant correlation was seen with other clinicopathological factors. These results suggest that FGF-2 may play an important role for the growth of diffuse type gastric cancers, particularly at their advanced stage.

MeSH terms

  • Aged
  • Biomarkers, Tumor / metabolism*
  • Disease Progression
  • Female
  • Fibroblast Growth Factor 2 / genetics
  • Fibroblast Growth Factor 2 / metabolism*
  • Humans
  • In Situ Hybridization
  • Male
  • Neoplasm Staging
  • RNA, Messenger / metabolism*
  • Stomach Neoplasms / metabolism*
  • Stomach Neoplasms / mortality
  • Stomach Neoplasms / pathology
  • Survival Rate

Substances

  • Biomarkers, Tumor
  • RNA, Messenger
  • Fibroblast Growth Factor 2