Structural study of the O-linked sugar chains of human leukocyte tyrosine phosphatase CD45

Eur J Biochem. 1998 Jan 15;251(1-2):288-94. doi: 10.1046/j.1432-1327.1998.2510288.x.


The O-linked sugar chains of the human leukocyte cell surface glycoprotein CD45 were released as tritium-labeled oligosaccharides by beta-elimination in the presence of NaB3H4. Mono Q column chromatography revealed that they comprise neutral (64%) and acidic (36%) oligosaccharides, the latter of which were converted to neutral ones by Arthrobacter ureafaciens sialidase treatment. Structural studies of each oligosaccharide fractionated on a Bio-Gel P-4 column by sequential exoglycosidase digestion and by methylation analysis revealed that human leukocyte CD45 contains mainly core 1 and core 2 oligosaccharides, 15% of which are modified with poly (N-acetyllactosamine) chains in different extensions. CD45 consists of several isoforms which were isolated after cell surface sialic acid residues were labeled by periodate/NaB3H4 treatment. Bio-Gel P-6 column chromatography of a mixture of the tritium-labeled glycopeptide/oligosaccharides obtained by pronase-digestion followed by mild alkaline borohydride treatment showed that distribution of the sialylated core 2 oligosaccharides is different among CD45 isoforms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, Liquid / methods
  • Humans
  • Leukocyte Common Antigens / chemistry*
  • Leukocytes / chemistry
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*


  • Oligosaccharides
  • Leukocyte Common Antigens