Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes

Arch Microbiol. 1998 Aug;170(2):85-90. doi: 10.1007/s002030050618.


A bacterial strain capable of utilizing E-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as Rhodococcus sp. The strain contained a novel aldoxime dehydration activity that catalyzed a stoichiometric dehydration of E-pyridine-3-aldoxime to form 3-cyanopyridine. The enzyme activity was induced by various aldoximes and nitriles. The strain metabolized the aldoxime as follows: E-pyridine-3-aldoxime was dehydrated to form 3-cyanopyridine, which was converted to nicotinamide by a nitrile hydratase, and the nicotinamide was successively hydrolyzed to nicotinic acid by an amidase.

MeSH terms

  • Amidohydrolases / metabolism
  • Bacterial Proteins / metabolism
  • Enzyme Induction / physiology
  • Kinetics
  • Niacinamide / metabolism
  • Nitriles / metabolism*
  • Oximes / metabolism*
  • Pyridines / metabolism*
  • Rhodococcus / enzymology*
  • Soil Microbiology


  • Bacterial Proteins
  • Nitriles
  • Oximes
  • Pyridines
  • Niacinamide
  • Amidohydrolases
  • 3-cyanopyridine