Bioremediation of epsilon-caprolactam from nylon-6 waste water by use of Pseudomonas aeruginosa MCM B-407

Curr Microbiol. 1998 Sep;37(3):191-4. doi: 10.1007/s002849900362.


Nylon-6, a man-made polymer that finds its application in the manufacture of car tires, ropes, fabrics, automobile parts etc., is manufactured with epsilon-caprolactam. Waste water generated during production of nylon-6 contains the unreacted monomer. Owing to the polluting and toxic nature of epsilon-caprolactam, its removal from waste streams is necessary. Pseudomonas aeruginosa MCM B-407 was isolated from activated sludge used to treat waste from a factory producing nylon-6. This organism was able to remove epsilon-caprolactam with simultaneous reduction in chemical oxygen demand (COD). The degradation of epsilon-caprolactam in waste water was found to be optimal over a wide range of pH from 5.0 to 9.0, temperature of 30 degrees C, and under shake or aerated conditions, with an inoculum density of 10(5) cells/ml and with an incubation period of 24 - 48 h. Thus, Pseudomonas aeruginosa MCM B-407 isolated from the activated sludge exposed to epsilon-caprolactam may play an important role in the bioremediation of epsilon-caprolactam from the waste waters of industries manufacturing nylon-6.

MeSH terms

  • Caprolactam / analogs & derivatives*
  • Caprolactam / metabolism*
  • Hydrogen-Ion Concentration
  • Industrial Waste*
  • Pseudomonas aeruginosa / isolation & purification
  • Pseudomonas aeruginosa / metabolism*
  • Temperature
  • Time Factors
  • Water Pollution*


  • Industrial Waste
  • Caprolactam