The Trp64Arg polymorphism of the beta 3-adrenergic receptor gene is not associated with obesity or type 2 diabetes mellitus in a large population-based Caucasian cohort

J Clin Endocrinol Metab. 1998 Aug;83(8):2892-7. doi: 10.1210/jcem.83.8.5004.


The beta3-adrenergic receptor (3-BAR) is assumed to play a role in the regulation of energy balance by increasing lipolysis and thermogenesis. A recently detected allelic polymorphism (Trp64Arg polymorphism) has been suggested to contribute to the development of obesity and non-insulin-dependent diabetes mellitus. We examined the prevalence of the two 3-BAR alleles in Germany and looked for associations between 3-BAR genotype and metabolic disorders (obesity and type 2 diabetes mellitus). From over 6450 participants in the Diabetomobile Study, a nationwide epidemiologic study on the prevalence of metabolic disorders (carried out from 1993 to 1996 in Germany), 1259 participants were randomly chosen. The 3-BAR genotype status was determined by 3-BAR gene-specific genomic PCR and consecutive restriction fragment length polymorphism analysis. The frequencies of the different genotypes in the examined cohort were as follows: Trp64/Trp64, 88.3%; Trp64/Arg64, 10.8%; and Arg64/ Arg64, 0.8%. No significant differences between the different genotypes were found when comparing age, body mass index, weight, total and high-density lipoprotein (HDL) cholesterol, fasting insulin, HbA11, and blood pressure; neither did the type 2 diabetes mellitus participants in the different genotype groups differ significantly in terms of age of diabetes onset or HbA11. This is the largest population-based study on the Trp64Arg polymorphism reported yet. The Arg64 allele of the 3-BAR gene was found commonly in Germany. In our cohort, no significant associations between the Arg64 allele and metabolic disorders (e.g. obesity, type 2 diabetes mellitus, dyslipidemia, or hypertension) were detected.

MeSH terms

  • Adult
  • Aging
  • Alleles
  • Arginine / genetics*
  • Body Mass Index
  • Body Temperature Regulation
  • Body Weight
  • Cholesterol / blood
  • Cohort Studies
  • Diabetes Mellitus, Type 1 / genetics
  • Diabetes Mellitus, Type 2 / genetics*
  • Energy Metabolism
  • Female
  • Genotype
  • Humans
  • Lipolysis
  • Male
  • Middle Aged
  • Obesity / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • Random Allocation
  • Receptors, Adrenergic, beta / genetics*
  • Tryptophan / genetics*


  • Receptors, Adrenergic, beta
  • Tryptophan
  • Arginine
  • Cholesterol