Two histidine residues are essential for ribonuclease T1 activity as is the case for ribonuclease A.
Nishikawa S, Morioka H, Kim HJ, Fuchimura K, Tanaka T, Uesugi S, Hakoshima T, Tomita K, Ohtsuka E, Ikehara M.
Nishikawa S, et al. Among authors: ikehara m.
Biochemistry. 1987 Dec 29;26(26):8620-4. doi: 10.1021/bi00400a019.
Biochemistry. 1987.
PMID: 3126807
., Morioka, H., Fuchimura, K., Tanaka, T., Uesugi, S., Ohtsuka, E., & Ikehara, M. (1986) Biochem. Biophys. Res. Commun. 138, 789-794]. In the present paper, we report that mutants of RNase T1 with residue Ala-40 or Ala-92 have almost no activity, while mutants t …
., Morioka, H., Fuchimura, K., Tanaka, T., Uesugi, S., Ohtsuka, E., & Ikehara, M. (1986) Biochem. Biophys. Res. Commun. 13 …