Structural and thermodynamic analyses of Escherichia coli RNase HI variant with quintuple thermostabilizing mutations.
Haruki M, Tanaka M, Motegi T, Tadokoro T, Koga Y, Takano K, Kanaya S.
Haruki M, et al. Among authors: kanaya s.
FEBS J. 2007 Nov;274(22):5815-25. doi: 10.1111/j.1742-4658.2007.06104.x. Epub 2007 Oct 18.
FEBS J. 2007.
PMID: 17944939
Free article.
A combination of five thermostabilizing mutations, Gly23-->Ala, His62-->Pro, Val74-->Leu, Lys95-->Gly, and Asp134-->His, has been shown to additively enhance the thermostability of Escherichia coli RNase HI [Akasako A, Haruki M, Oobatake M & Kanaya S …
A combination of five thermostabilizing mutations, Gly23-->Ala, His62-->Pro, Val74-->Leu, Lys95-->Gly, and Asp134-->His, has …