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Increased enantioselectivity by engineering bottleneck mutants in an esterase from Pseudomonas fluorescens.
Chembiochem. 2009 Dec 14;10(18):2920-3. doi: 10.1002/cbic.200900563.
Chembiochem. 2009.
PMID: 19847842
A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution.
Hidalgo A, Schliessmann A, Molina R, Hermoso J, Bornscheuer UT.
Hidalgo A, et al. Among authors: schliessmann a.
Protein Eng Des Sel. 2008 Sep;21(9):567-76. doi: 10.1093/protein/gzn034. Epub 2008 Jun 17.
Protein Eng Des Sel. 2008.
PMID: 18559369
Free article.
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Promiscuous enantioselective (-)-γ-lactamase activity in the Pseudomonas fluorescens esterase I.
Torres LL, Schliessmann A, Schmidt M, Silva-Martin N, Hermoso JA, Berenguer J, Bornscheuer UT, Hidalgo A.
Torres LL, et al. Among authors: schliessmann a.
Org Biomol Chem. 2012 May 7;10(17):3388-92. doi: 10.1039/c2ob06887g. Epub 2012 Feb 22.
Org Biomol Chem. 2012.
PMID: 22359066
Free article.
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One-pot Simple methodology for CAssette Randomization and Recombination for focused directed evolution (OSCARR).
Hidalgo A, Schließmann A, Bornscheuer UT.
Hidalgo A, et al. Among authors: schliessmann a.
Methods Mol Biol. 2014;1179:207-12. doi: 10.1007/978-1-4939-1053-3_14.
Methods Mol Biol. 2014.
PMID: 25055780
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