Separation and Detection of Phosphorylated and Nonphosphorylated BvgA, a Bordetella pertussis Response Regulator, in vivo and in vitro.
Chen Q, Boulanger A, Hinton DM, Stibitz S.
Chen Q, et al. Among authors: boulanger a.
Bio Protoc. 2013;3(22):e970. doi: 10.21769/BioProtoc.970.
Bio Protoc. 2013.
PMID: 27747260
Free PMC article.
Here we describe a method to detect phosphorylation of the Bordetella pertussis response regulator BvgA, which is phosphorylated at an aspartate residue (Boulanger et al., 2013). This method is based on the proprietary adduct, Phos-tag, a dinuclear metal comp …
Here we describe a method to detect phosphorylation of the Bordetella pertussis response regulator BvgA, which is phosphorylated at a …