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Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.
Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino S, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, Gromek KA, Seder KD, Hwang S, Primm JG, Sabat G, Vojtik FC, Volkman BF, Zolnai Z, Phillips GN Jr, Markley JL, Fox BG. Aceti DJ, et al. Among authors: bergeman lf. J Struct Funct Genomics. 2015 Jun;16(2):67-80. doi: 10.1007/s10969-015-9198-1. Epub 2015 Apr 9. J Struct Funct Genomics. 2015. PMID: 25854603 Free PMC article.
Small-scale, semi-automated purification of eukaryotic proteins for structure determination.
Frederick RO, Bergeman L, Blommel PG, Bailey LJ, McCoy JG, Song J, Meske L, Bingman CA, Riters M, Dillon NA, Kunert J, Yoon JW, Lim A, Cassidy M, Bunge J, Aceti DJ, Primm JG, Markley JL, Phillips GN Jr, Fox BG. Frederick RO, et al. J Struct Funct Genomics. 2007 Dec;8(4):153-66. doi: 10.1007/s10969-007-9032-5. Epub 2007 Nov 6. J Struct Funct Genomics. 2007. PMID: 17985212 Free PMC article.
Cell-free translation of biofuel enzymes.
Takasuka TE, Walker JA, Bergeman LF, Vander Meulen KA, Makino S, Elsen NL, Fox BG. Takasuka TE, et al. Among authors: bergeman lf. Methods Mol Biol. 2014;1118:71-95. doi: 10.1007/978-1-62703-782-2_5. Methods Mol Biol. 2014. PMID: 24395410 Free PMC article.
Active site and laminarin binding in glycoside hydrolase family 55.
Bianchetti CM, Takasuka TE, Deutsch S, Udell HS, Yik EJ, Bergeman LF, Fox BG. Bianchetti CM, et al. Among authors: bergeman lf. J Biol Chem. 2015 May 8;290(19):11819-32. doi: 10.1074/jbc.M114.623579. Epub 2015 Mar 9. J Biol Chem. 2015. PMID: 25752603 Free PMC article.
Development of a High Throughput Platform for Screening Glycoside Hydrolases Based on Oxime-NIMS.
Deng K, Guenther JM, Gao J, Bowen BP, Tran H, Reyes-Ortiz V, Cheng X, Sathitsuksanoh N, Heins R, Takasuka TE, Bergeman LF, Geertz-Hansen H, Deutsch S, Loqué D, Sale KL, Simmons BA, Adams PD, Singh AK, Fox BG, Northen TR. Deng K, et al. Among authors: bergeman lf. Front Bioeng Biotechnol. 2015 Oct 13;3:153. doi: 10.3389/fbioe.2015.00153. eCollection 2015. Front Bioeng Biotechnol. 2015. PMID: 26528471 Free PMC article.
14 results