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A short translational ramp determines the efficiency of protein synthesis.
Verma M, Choi J, Cottrell KA, Lavagnino Z, Thomas EN, Pavlovic-Djuranovic S, Szczesny P, Piston DW, Zaher HS, Puglisi JD, Djuranovic S. Verma M, et al. Among authors: choi j. Nat Commun. 2019 Dec 18;10(1):5774. doi: 10.1038/s41467-019-13810-1. Nat Commun. 2019. PMID: 31852903 Free PMC article.
Fluorescently-tagged human eIF3 for single-molecule spectroscopy.
Johnson AG, Petrov AN, Fuchs G, Majzoub K, Grosely R, Choi J, Puglisi JD. Johnson AG, et al. Among authors: choi j. Nucleic Acids Res. 2018 Jan 25;46(2):e8. doi: 10.1093/nar/gkx1050. Nucleic Acids Res. 2018. PMID: 29136179 Free PMC article.
Three tRNAs on the ribosome slow translation elongation.
Choi J, Puglisi JD. Choi J, et al. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13691-13696. doi: 10.1073/pnas.1719592115. Epub 2017 Dec 11. Proc Natl Acad Sci U S A. 2017. PMID: 29229848 Free PMC article.
2'-O-methylation in mRNA disrupts tRNA decoding during translation elongation.
Choi J, Indrisiunaite G, DeMirci H, Ieong KW, Wang J, Petrov A, Prabhakar A, Rechavi G, Dominissini D, He C, Ehrenberg M, Puglisi JD. Choi J, et al. Nat Struct Mol Biol. 2018 Mar;25(3):208-216. doi: 10.1038/s41594-018-0030-z. Epub 2018 Feb 19. Nat Struct Mol Biol. 2018. PMID: 29459784 Free PMC article.
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