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Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases.
Nucleic Acids Res. 2020 Apr 17;48(7):3722-3733. doi: 10.1093/nar/gkaa110.
Nucleic Acids Res. 2020.
PMID: 32107556
Free PMC article.
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning.
Tóth E, Huszár K, Bencsura P, Kulcsár PI, Vodicska B, Nyeste A, Welker Z, Tóth S, Welker E.
Tóth E, et al. Among authors: welker z.
PLoS One. 2014 Mar 11;9(3):e90896. doi: 10.1371/journal.pone.0090896. eCollection 2014.
PLoS One. 2014.
PMID: 24618593
Free PMC article.
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Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs.
Kulcsár PI, Tálas A, Tóth E, Nyeste A, Ligeti Z, Welker Z, Welker E.
Kulcsár PI, et al. Among authors: welker z.
Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5.
Nat Commun. 2020.
PMID: 32144253
Free PMC article.
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A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs.
Tálas A, Huszár K, Kulcsár PI, Varga JK, Varga É, Tóth E, Welker Z, Erdős G, Pach PF, Welker Á, Györgypál Z, Tusnády GE, Welker E.
Tálas A, et al. Among authors: welker z.
Nucleic Acids Res. 2021 Apr 6;49(6):e31. doi: 10.1093/nar/gkaa1220.
Nucleic Acids Res. 2021.
PMID: 33450024
Free PMC article.
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Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs.
Huszár K, Welker Z, Györgypál Z, Tóth E, Ligeti Z, Kulcsár PI, Dancsó J, Tálas A, Krausz SL, Varga É, Welker E.
Huszár K, et al. Among authors: welker z.
Nucleic Acids Res. 2023 Jun 23;51(11):5847-5863. doi: 10.1093/nar/gkad323.
Nucleic Acids Res. 2023.
PMID: 37140059
Free PMC article.
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