An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding.
Dürrschmidt P, Mansfeld J, Ulbrich-Hofmann R.
Dürrschmidt P, et al. Among authors: mansfeld j.
FEBS J. 2005 Mar;272(6):1523-34. doi: 10.1111/j.1742-4658.2005.04593.x.
FEBS J. 2005.
PMID: 15752367
Free article.
The extreme thermal stabilization achieved by the introduction of a disulfide bond (G8C/N60C) into the cysteine-free wild-type-like mutant (pWT) of the neutral protease from Bacillus stearothermophilus[Mansfeld J, Vriend G, Dijkstra BW, Veltman OR, Van den Burg B, V …
The extreme thermal stabilization achieved by the introduction of a disulfide bond (G8C/N60C) into the cysteine-free wild-type-like mutant ( …