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An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding.
Dürrschmidt P, Mansfeld J, Ulbrich-Hofmann R. Dürrschmidt P, et al. Among authors: mansfeld j. FEBS J. 2005 Mar;272(6):1523-34. doi: 10.1111/j.1742-4658.2005.04593.x. FEBS J. 2005. PMID: 15752367 Free article.
The extreme thermal stabilization achieved by the introduction of a disulfide bond (G8C/N60C) into the cysteine-free wild-type-like mutant (pWT) of the neutral protease from Bacillus stearothermophilus[Mansfeld J, Vriend G, Dijkstra BW, Veltman OR, Van den Burg B, V …
The extreme thermal stabilization achieved by the introduction of a disulfide bond (G8C/N60C) into the cysteine-free wild-type-like mutant ( …
Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus stearothermophilus containing an engineered disulfide bond.
Dürrschmidt P, Mansfeld J, Ulbrich-Hofmann R. Dürrschmidt P, et al. Among authors: mansfeld j. Eur J Biochem. 2001 Jun;268(12):3612-8. doi: 10.1046/j.1432-1327.2001.02270.x. Eur J Biochem. 2001. PMID: 11422393 Free article.
The introduction of a disulfide bond into the neutral protease from Bacillus stearothermophilus by the double mutation G8C/N60C had resulted in an extremely thermostable enzyme with a half-life of 35.9 min at 92.5 degrees C [Mansfeld, J., Vriend, G., Dijkstra, B.W., …
The introduction of a disulfide bond into the neutral protease from Bacillus stearothermophilus by the double mutation G8C/N60C had resulted …
79 results