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A High-Throughput Assay for In Vitro Determination of Release Factor-Dependent Peptide Release from a Pretermination Complex by Fluorescence Anisotropy-Application to Nonsense Suppressor Screening and Mechanistic Studies.
Biomolecules. 2023 Jan 27;13(2):242. doi: 10.3390/biom13020242.
Biomolecules. 2023.
PMID: 36830611
Free PMC article.
Ataluren binds to multiple protein synthesis apparatus sites and competitively inhibits release factor-dependent termination.
Huang S, Bhattacharya A, Ghelfi MD, Li H, Fritsch C, Chenoweth DM, Goldman YE, Cooperman BS.
Huang S, et al. Among authors: ghelfi md.
Nat Commun. 2022 May 6;13(1):2413. doi: 10.1038/s41467-022-30080-6.
Nat Commun. 2022.
PMID: 35523781
Free PMC article.
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Ataluren and aminoglycosides stimulate read-through of nonsense codons by orthogonal mechanisms.
Ng MY, Li H, Ghelfi MD, Goldman YE, Cooperman BS.
Ng MY, et al. Among authors: ghelfi md.
Proc Natl Acad Sci U S A. 2021 Jan 12;118(2):e2020599118. doi: 10.1073/pnas.2020599118.
Proc Natl Acad Sci U S A. 2021.
PMID: 33414181
Free PMC article.
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