Protein engineering of Bacillus thermocatenulatus lipase via deletion of the α5 helix.
Goodarzi N, Karkhane AA, Mirlohi A, Tabandeh F, Torktas I, Aminzadeh S, Yakhchali B, Shamsara M, Ghafouri MA.
Goodarzi N, et al.
Appl Biochem Biotechnol. 2014 Sep;174(1):339-51. doi: 10.1007/s12010-014-1063-3. Epub 2014 Jul 27.
Appl Biochem Biotechnol. 2014.
PMID: 25064134
Lipases from Bacillus thermocatenulatus are a member of superfamily of alpha/beta hydrolase, but there are structural differences between them. In this work, we focused on the alpha5 helix of B. thermocatenulatus lipase (BTL2) which is absent in canonical alpha/beta hydrol …
Lipases from Bacillus thermocatenulatus are a member of superfamily of alpha/beta hydrolase, but there are structural differences between th …