Selection of single-chain variable fragments specific for Mycobacterium tuberculosis ESAT-6 antigen using ribosome display.
Ahangarzadeh S, Bandehpour M, Kazemi B.
Ahangarzadeh S, et al.
Iran J Basic Med Sci. 2017 Mar;20(3):327-333. doi: 10.22038/ijbms.2017.8363.
Iran J Basic Med Sci. 2017.
PMID: 28392906
Free PMC article.
The purified recombinant ESAT-6 protein was injected into female BALB/c mice for immunization, and then m-RNA was extracted from the spleen of immunized mice. The anti-ESAT-6 VH/k chain library was assembled by joining of VH and k into the VH/k chain with a 72-bp DNA linke …
The purified recombinant ESAT-6 protein was injected into female BALB/c mice for immunization, and then m-RNA was extracted from the …