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The effect of female sex steroid hormones on osteogenic differentiation of endometrial stem cells.
Soltanyzadeh M, Salimi A, Halabian R, Ghollasi M. Soltanyzadeh M, et al. Mol Biol Rep. 2020 May;47(5):3663-3674. doi: 10.1007/s11033-020-05461-z. Epub 2020 Apr 25. Mol Biol Rep. 2020. PMID: 32335804
For this purpose, hEnSCs were treated with 17-beta estradiol and progesterone separately (1 10(-6) M) and simultaneously (1 10(-7) M). Osteogenic differentiation tests including measurement of total mineral calcium content, Alizarin Red staining, the quantitative ex …
For this purpose, hEnSCs were treated with 17-beta estradiol and progesterone separately (1 10(-6) M) and simultaneously (1 10(-7) …
Oxaloacetate as new inducer for osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells in vitro.
Shirkoohi FJ, Ghollasi M, Halabian R, Eftekhari E, Ghiasi M. Shirkoohi FJ, et al. Mol Biol Rep. 2024 Mar 27;51(1):451. doi: 10.1007/s11033-024-09389-6. Mol Biol Rep. 2024. PMID: 38536507
Various assays including alkaline phosphatase assay (ALP), cellular calcium content assay, mineralized matrix staining with alizarin red, catalase (CAT) and superoxide dismutase (SOD) activity, and real-time RT-PCR analysis of three bone-specific markers (ALP, osteocalcin, and co …
Various assays including alkaline phosphatase assay (ALP), cellular calcium content assay, mineralized matrix staining with alizarin red, ca …
A comparative study of hBM-MSCs' differentiation toward osteogenic lineage in the presence of progesterone and estrogen hormones separately and concurrently in vitro.
Soltanyzadeh M, Ghollasi M, Halabian R, Shams M. Soltanyzadeh M, et al. Cell Biol Int. 2020 Aug;44(8):1701-1713. doi: 10.1002/cbin.11364. Epub 2020 May 6. Cell Biol Int. 2020. PMID: 32339349
Also, sex hormones act as important elements in bone development and maintenance, and the roles of two female sex steroid hormones known as estrogen (17-beta estradiol) and progesterone in osteogenic differentiation of human BM-MSCs (hBM-MSCs) are studied. For this purpose, hBM-M …
Also, sex hormones act as important elements in bone development and maintenance, and the roles of two female sex steroid hormones known as …
Engineering of a Bacillus alpha-amylase with improved thermostability and calcium independency.
Ghollasi M, Khajeh K, Naderi-Manesh H, Ghasemi A. Ghollasi M, et al. Appl Biochem Biotechnol. 2010 Sep;162(2):444-59. doi: 10.1007/s12010-009-8879-2. Epub 2010 Feb 23. Appl Biochem Biotechnol. 2010. PMID: 20177823
In comparison to the wild type, calcium ion had more effect on the catalytic efficiency, k (cat)/K (m), and half-life (at 60 degrees C) of A53S mutant. In A53S, the dependence of half-life on calcium concentration showed that the enhanced calcium binding is likely to be re …
In comparison to the wild type, calcium ion had more effect on the catalytic efficiency, k (cat)/K (m), and half-life (at 60 degrees …