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Triage of human papillomavirus infected women by methylation analysis in first-void urine.
Van Keer S, van Splunter AP, Pattyn J, De Smet A, Herzog SA, Van Ostade X, Tjalma WAA, Ieven M, Van Damme P, Steenbergen RDM, Vorsters A. Van Keer S, et al. Sci Rep. 2021 Apr 12;11(1):7862. doi: 10.1038/s41598-021-87329-1. Sci Rep. 2021. PMID: 33846517 Free PMC article.
Cytology, histology, and HPV DNA genotyping results on paired FVU and cervical samples were available. Urinary median methylation levels from HR-HPV (n = 93) positive women were found to increase for all markers with severity of underlying disease. Significantly elevated l …
Cytology, histology, and HPV DNA genotyping results on paired FVU and cervical samples were available. Urinary median methylation levels fro …
Identification of protein biomarkers for cervical cancer using human cervicovaginal fluid.
Van Raemdonck GA, Tjalma WA, Coen EP, Depuydt CE, Van Ostade XW. Van Raemdonck GA, et al. PLoS One. 2014 Sep 12;9(9):e106488. doi: 10.1371/journal.pone.0106488. eCollection 2014. PLoS One. 2014. PMID: 25215525 Free PMC article.
RESULTS: The proteome analysis revealed 16 candidate biomarkers of which alpha-actinin-4 (p = 0.001) and pyruvate kinase isozyme M1/M2 (p = 0.014) were most promising. Verification of alpha-actinin-4 by ELISA (n = 28) showed that this candidate biomarker discriminated betw …
RESULTS: The proteome analysis revealed 16 candidate biomarkers of which alpha-actinin-4 (p = 0.001) and pyruvate kinase isozyme M1/M2 (p = …
First-void urine as a non-invasive liquid biopsy source to detect vaccine-induced human papillomavirus antibodies originating from cervicovaginal secretions.
Van Keer S, Willhauck-Fleckenstein M, Pattyn J, Butt J, Tjalma WAA, Van Ostade X, Hens N, Van Damme P, Waterboer T, Vorsters A. Van Keer S, et al. J Clin Virol. 2019 Aug;117:11-18. doi: 10.1016/j.jcv.2019.05.004. Epub 2019 May 11. J Clin Virol. 2019. PMID: 31129514 Free article.
STUDY DESIGN: In this case-control study, 55 paired first-void urine and serum samples were included from 19- to 26-year-old women, unvaccinated (n = 19) or vaccinated (n = 36) with the bi- or quadrivalent HPV vaccine during adolescence (NCT02714114). ...
STUDY DESIGN: In this case-control study, 55 paired first-void urine and serum samples were included from 19- to 26-year-old women, unvaccin …
Supplementing formula-fed piglets with a low molecular weight fraction of bovine colostrum whey results in an improved intestinal barrier.
De Vos M, Huygelen V, Van Raemdonck G, Willemen S, Fransen E, Van Ostade X, Casteleyn C, Van Cruchten S, Van Ginneken C. De Vos M, et al. J Anim Sci. 2014 Aug;92(8):3491-501. doi: 10.2527/jas.2013-6437. Epub 2014 Jul 10. J Anim Sci. 2014. PMID: 25012977 Clinical Trial.
To test the hypothesis that a low molecular weight fraction of colostral whey could affect the morphology and barrier function of the small intestine, 30 3-d-old piglets (normal or low birth weight) were suckled (n = 5), artificially fed with milk formula (n = 5), o …
To test the hypothesis that a low molecular weight fraction of colostral whey could affect the morphology and barrier function of the small …
Needle lost in the haystack: multiple reaction monitoring fails to detect Treponema pallidum candidate protein biomarkers in plasma and urine samples from individuals with syphilis.
Van Raemdonck GA, Osbak KK, Van Ostade X, Kenyon CR. Van Raemdonck GA, et al. F1000Res. 2018 Mar 19;7:336. doi: 10.12688/f1000research.13964.2. eCollection 2018. F1000Res. 2018. PMID: 30519456 Free PMC article.
Thirty isotopically labelled proteotypic surrogate peptides (hPTPs) were synthesized and incorporated into a scheduled multiple reaction monitoring assay. Protein extracts from undepleted/unenriched plasma (N = 18) and urine (N = 4) samples from 18 individuals with …
Thirty isotopically labelled proteotypic surrogate peptides (hPTPs) were synthesized and incorporated into a scheduled multiple reaction mon …
Human papillomavirus genotype and viral load agreement between paired first-void urine and clinician-collected cervical samples.
Van Keer S, Tjalma WAA, Pattyn J, Biesmans S, Pieters Z, Van Ostade X, Ieven M, Van Damme P, Vorsters A. Van Keer S, et al. Eur J Clin Microbiol Infect Dis. 2018 May;37(5):859-869. doi: 10.1007/s10096-017-3179-1. Epub 2018 Feb 7. Eur J Clin Microbiol Infect Dis. 2018. PMID: 29417310 Free PMC article.
A higher high-risk HPV DNA prevalence was observed in first-void urine (n = 76/110) compared to cervical samples (n = 73/110), with HPV31 and HPV16/31 being most prevalent correspondingly. ...
A higher high-risk HPV DNA prevalence was observed in first-void urine (n = 76/110) compared to cervical samples (n = 73/110), …
The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome.
Gilany K, Van Elzen R, Mous K, Coen E, Van Dongen W, Vandamme S, Gevaert K, Timmerman E, Vandekerckhove J, Dewilde S, Van Ostade X, Moens L. Gilany K, et al. Biochim Biophys Acta. 2008 Jul-Aug;1784(7-8):983-5. doi: 10.1016/j.bbapap.2008.03.003. Epub 2008 Mar 19. Biochim Biophys Acta. 2008. PMID: 18402783
A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. ...
A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, …