oFlowSeq: a quantitative approach to identify protein coding mutations affecting cell type enrichment using mosaic CRISPR-Cas9 edited cerebral organoids.
Dawes P, Murray LF, Olson MN, Barton NJ, Smullen M, Suresh M, Yan G, Zhang Y, Fernandez-Fontaine A, English J, Uddin M, Pak C, Church GM, Chan Y, Lim ET.
Dawes P, et al.
Hum Genet. 2023 Aug;142(8):1281-1291. doi: 10.1007/s00439-023-02534-4. Epub 2023 Mar 6.
Hum Genet. 2023.
PMID: 36877372
Free PMC article.
Using oFlowSeq, we found that deleterious mutations in autism-associated gene KCTD13 resulted in increased proportions of Nestin(+) cells and decreased proportions of TRA-1-60(+) cells within mosaic cerebral organoids. ...
Using oFlowSeq, we found that deleterious mutations in autism-associated gene KCTD13 resulted in increased proportions of Nestin(+) c …