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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1966 1
1970 1
1972 18
1973 39
1974 55
1975 46
1976 48
1977 68
1978 33
1979 109
1980 43
1981 42
1982 55
1983 60
1984 45
1985 45
1986 32
1987 44
1988 45
1989 49
1990 54
1991 47
1992 34
1993 52
1994 46
1995 43
1996 33
1997 28
1998 28
1999 24
2000 30
2001 24
2002 23
2003 22
2004 18
2005 20
2006 24
2007 41
2008 18
2009 17
2010 11
2011 22
2012 24
2013 13
2014 15
2015 6
2016 9
2017 10
2018 11
2019 3
2020 0
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1,601 results
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Page 1
A User's Guide for Phase Separation Assays with Purified Proteins.
Alberti S, et al. J Mol Biol 2018. PMID 29944854 Free PMC article.
In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. ...Thus, there is an urgent need for high-quality proteins, standardized procedures, and generally agreed-upon practices for protein purification and conducting phase separation assays. ...
In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membran …
Expression and purification of human ribosomal proteins S3, S5, S10, S19, and S26.
Malygin A, et al. Protein Expr Purif 2003. PMID 12651107
The cDNAs for the human ribosomal proteins S3, S5, S10, S19, and S26 were introduced into a pET-15b vector and recombinant proteins containing an N-(His)(6)-fusion tag were expressed in high yields. ...Complete purification of S3 required an additional gel-filtration step. The proteins were refolded by stepwise dialysis. Both identity and purity of the proteins were confirmed by 2D PAGE. ...
The cDNAs for the human ribosomal proteins S3, S5, S10, S19, and S26 were introduced into a pET-15b vector and recombinant …
Purification and characterization of the 30S ribosomal proteins from the bacterium Thermus thermophilus.
Tsiboli P, et al. Eur J Biochem 1994. PMID 7957245 Free article.
The proteins are numbered according to their primary structural similarity with known prokaryotic ribosomal proteins. Eight of them, namely proteins S6, S7, S9, S10, S14, S15, S16 and S17 run at different positions in the two-dimensional gel electrophoresis system to those suggested [Sedelnikova, S. ...All characterized proteins are homologous to known ribosomal proteins from other species, except for a small basic protein which shows homology only to a ribosomal protein from spinach chloroplasts [Choli, T., Franceschi, F., Yonath, A. & Wittmann-Leibold, B. (1993) Biol. ...
The proteins are numbered according to their primary structural similarity with known prokaryotic ribosomal proteins. E …
The complex between ribosomal proteins and aminoacyl-tRNA: the interactions and hydrolytic activities are not confined to the proteins L2 and L16 of Escherichia coli ribosomes.
Sumpter VG, et al. Biochim Biophys Acta 1990. PMID 2182127
The capacity of some Escherichia coli (E. coli) ribosomal proteins to bind to tRNA and to hydrolyse their aminoacylated derivatives has been analysed. The following results were obtained: (1) The basic proteins L2, L16 and L33 and S20 bound f[3H]Met-tRNA to a similar extent as the total proteins from 30 S (TP30) or 50 S (TP50) when tested by nitrocellulose filtration, in contrast to the more acidic proteins L7/L12 and S8. (2) The proteins of the peptidyltransferase centre, L2 and L16, showed no distinct specificity, binding various charged tRNAs from E. coli and Saccharomyces cerevisiae (S. cerevisiae). (3) A number of isolated ribosomal proteins hydrolysed aminoacyl-tRNA as assessed by trichloroacetic acid precipitation, in contrast to the TP30 and TP50. (4) The loss of radiolabel from Ac[14C]Phe-tRNA and from [14C]tRNA in the presence of these proteins could not be prevented by RNasin, a ribonuclease inhibitor, whereas that mediated by a sample of non-RNase-free bovine serum albumin was inhibited. (5) When double-labelled, Ac[3H]Phe-[14C]tRNA was incubated with L2 both radiolabels were lost, indicating that this potential candidate for a peptidyltransferase enzyme does not specifically cleave the ester bond between the aminoacyl residue and the tRNA....
The capacity of some Escherichia coli (E. coli) ribosomal proteins to bind to tRNA and to hydrolyse their aminoacylated deriva …
Isolation of eukaryotic ribosomal proteins. Purification and characterization of the 60 S ribosomal subunit proteins La, Lb, Lf, P1, P2, L13', L14, L18', L20, and L38.
Tsurugi K, et al. J Biol Chem 1978. PMID 621213 Free article.
Two of the proteins (La and L18') had no detectable contamination; the impurities in the others were not greater than 8%. The molecular weight of the proteins was estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate; the amino acid composition was determined. ...P1 and P2 are distinct proteins but both have large amounts of alanine (20.4 and 17.5 mol %)....
Two of the proteins (La and L18') had no detectable contamination; the impurities in the others were not greater than 8%. The molecul …
Characterization of Nicotiana tabacum chloroplast and cytoplasmic ribosomal proteins.
Capel MS and Bourque DP. J Biol Chem 1982. PMID 7085647 Free article.
Average absolute mobility maps, constructed from the resultant electropherograms, were used to compare ribosomal proteins from cytoplasmic and chloroplast ribosomes. ...These data substantiate: 1) the close affiliation between higher plant chloroplast and prokaryotic ribosomes and 2) a general similarity between angiosperm cytoplasmic ribosomal proteins and other classes of eukaryotic cytoplasmic ribosomal proteins....
Average absolute mobility maps, constructed from the resultant electropherograms, were used to compare ribosomal proteins from …
Isolation and amino acid sequence of the 30S ribosomal protein S19 from Mycobacterium bovis BCG.
Ohara N, et al. FEBS Lett 1993. PMID 8405418 Free article.
The 30S ribosomal proteins from Mycobacterium bovis BCG were separated by reverse phase-high performance liquid chromatography (RP-HPLC). ...This sequence proved to be 64% and 71% identical to those of the corresponding S19 proteins from the eubacteria E. coli, and B. stearothermophilus, respectively; 33% of the residues of MboS19 were identical to those in the archaebacterial ribosomal protein HmaS19....
The 30S ribosomal proteins from Mycobacterium bovis BCG were separated by reverse phase-high performance liquid chromatography …
Evolutionary microdivergence of chick and rat liver ribosomal proteins.
Ramjoué HP and Gordon J. J Biol Chem 1977. PMID 925036 Free article.
We have set out to establish a nomenclature for ribosomal proteins which transcends, as far as is possible, the species from which the ribosomes were isolated. ...We carried out a detailed comparison of the rat liver proteins with those of chick liver; parallel separate and mixed preparations of ribosomal subunits from the two species were made. ...
We have set out to establish a nomenclature for ribosomal proteins which transcends, as far as is possible, the species from w …
Studies on ribosomal proteins in the cellular slime mold Dictyostelium discoideum. Resolution, nomenclature and molecular weights of proteins in the 40-S and 60-S ribosomal subunits.
Ramagopal S and Ennis HL. Eur J Biochem 1980. PMID 7379784 Free article.
This study is concerned with the identification and subunit localization of ribosomal proteins in Dictyostelium discoideum. The characterization is based on the resolution of ribosomal proteins by various methods of electrophoresis. 34 and 42 unique proteins were identified in the 40-S and 60-S ribosomal subunits respectively. ...Purification of ribosomes in high-ionic-strength buffers resulted in non-specific loss of the various proteins from the 40-S and 60-S subunits. ...
This study is concerned with the identification and subunit localization of ribosomal proteins in Dictyostelium discoideum. Th …
Purification of Drosophila acidic ribosomal proteins.
Chooi WY, et al. Eur J Biochem 1982. PMID 6814910 Free article.
Five proteins (7/8, S25/S27, S14, L1/L2 and L5/L6) required no further purification. The others were further purified as follows: proteins S7, and S9 by preparative gel electrophoresis: and protein 13 (to newly identified protein) by adsorption with conconavalin-A--agarose. Four proteins had no detectable contamination, and in each of the others the impurities were no greater than 3%. The amount of purified protein recovered from a starting amount of 2.63 g total 80-S ribosomal protein and a starting amount of 105 mg group A80 varied from 0.4 mg to 8.8 mg. ...
Five proteins (7/8, S25/S27, S14, L1/L2 and L5/L6) required no further purification. The others were further purified as follo …
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