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The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments.
Huggett JF, Foy CA, Benes V, Emslie K, Garson JA, Haynes R, Hellemans J, Kubista M, Mueller RD, Nolan T, Pfaffl MW, Shipley GL, Vandesompele J, Wittwer CT, Bustin SA. Huggett JF, et al. Among authors: vandesompele j. Clin Chem. 2013 Jun;59(6):892-902. doi: 10.1373/clinchem.2013.206375. Epub 2013 Apr 9. Clin Chem. 2013. PMID: 23570709
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating the measurement of small percentage differences and quan …
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordabl …
Digital PCR Partition Classification.
Vynck M, Chen Y, Gleerup D, Vandesompele J, Trypsteen W, Lievens A, Thas O, De Spiegelaere W. Vynck M, et al. Among authors: trypsteen w, vandesompele j, thas o, de spiegelaere w. Clin Chem. 2023 Sep 1;69(9):976-990. doi: 10.1093/clinchem/hvad063. Clin Chem. 2023. PMID: 37401391 Review.
BACKGROUND: Partition classification is a critical step in the digital PCR data analysis pipeline. A range of partition classification methods have been developed, many motivated by specific experimental setups. An overview of these partition classification methods …
BACKGROUND: Partition classification is a critical step in the digital PCR data analysis pipeline. A range of partition classi …
Digital PCR: a tool in clostridial mutant selection and detection.
Dierick E, Callens C, De Spiegelaere W, Ducatelle R, Van Immerseel F, Goossens E. Dierick E, et al. Among authors: de spiegelaere w. Appl Microbiol Biotechnol. 2023 Nov;107(22):6973-6983. doi: 10.1007/s00253-023-12779-8. Epub 2023 Sep 14. Appl Microbiol Biotechnol. 2023. PMID: 37704769
Since site-specific insertion of the mobile ClosTron insert may cause errors, validation is key. In this paper we describe the use of digital PCR (dPCR) as an alternative tool in selecting clostridial mutant strains. ...This research demonstrates that dPCR is indeed …
Since site-specific insertion of the mobile ClosTron insert may cause errors, validation is key. In this paper we describe the use of dig …
Digital PCR as a tool to measure HIV persistence.
Rutsaert S, Bosman K, Trypsteen W, Nijhuis M, Vandekerckhove L. Rutsaert S, et al. Among authors: trypsteen w. Retrovirology. 2018 Jan 30;15(1):16. doi: 10.1186/s12977-018-0399-0. Retrovirology. 2018. PMID: 29378600 Free PMC article. Review.
Most recently, digital PCR is gaining popularity as a novel approach to nucleic acid quantification as it allows for absolute target quantification. Various commercial digital PCR platforms are nowadays available that implement the principle of digi …
Most recently, digital PCR is gaining popularity as a novel approach to nucleic acid quantification as it allows for absolute …
Quality control of digital PCR assays and platforms.
Vynck M, Vandesompele J, Thas O. Vynck M, et al. Among authors: vandesompele j, thas o. Anal Bioanal Chem. 2017 Oct;409(25):5919-5931. doi: 10.1007/s00216-017-0538-9. Epub 2017 Aug 10. Anal Bioanal Chem. 2017. PMID: 28799053
Digital polymerase chain reaction (digital PCR, dPCR) is a direct nucleic acid quantification method, thus requiring no standard curves unlike quantitative real-time PCR (qPCR). ...
Digital polymerase chain reaction (digital PCR, dPCR) is a direct nucleic acid quantification method, thus requiring no …
Triplex digital PCR assays for the quantification of intact proviral HIV-1 DNA.
van Snippenberg W, Gleerup D, Rutsaert S, Vandekerckhove L, De Spiegelaere W, Trypsteen W. van Snippenberg W, et al. Among authors: trypsteen w, de spiegelaere w. Methods. 2022 May;201:41-48. doi: 10.1016/j.ymeth.2021.05.006. Epub 2021 May 13. Methods. 2022. PMID: 33992770
Therefore, an accurate evaluation and quantification of these (intact) proviruses is essential to determine the efficacy of HIV-1 cure strategies which aim to eliminate this reservoir. Here, we present two triplex digital PCR assays which resulted from a combination …
Therefore, an accurate evaluation and quantification of these (intact) proviruses is essential to determine the efficacy of HIV-1 cure strat …
Benchmarking digital PCR partition classification methods with empirical and simulated duplex data.
Chen Y, De Spiegelaere W, Trypsteen W, Gleerup D, Vandesompele J, Lievens A, Vynck M, Thas O. Chen Y, et al. Among authors: trypsteen w, vandesompele j, thas o, de spiegelaere w. Brief Bioinform. 2024 Mar 27;25(3):bbae120. doi: 10.1093/bib/bbae120. Brief Bioinform. 2024. PMID: 38555473 Free PMC article.
Digital PCR (dPCR) is a highly accurate technique for the quantification of target nucleic acid(s). ...
Digital PCR (dPCR) is a highly accurate technique for the quantification of target nucleic acid(s). ...
Digital PCR-based evaluation of nucleic acid extraction kit performance for the co-purification of cell-free DNA and RNA.
Deleu J, Schoofs K, Decock A, Verniers K, Roelandt S, Denolf A, Verreth J, De Wilde B, Van Maerken T, De Preter K, Vandesompele J. Deleu J, et al. Among authors: vandesompele j. Hum Genomics. 2022 Dec 31;16(1):73. doi: 10.1186/s40246-022-00446-4. Hum Genomics. 2022. PMID: 36587211 Free PMC article.
So far, no study has systematically compared the performance of cfDNA and cfRNA co-purification to increase sensitivity. RESULTS: First, we set up a framework using digital PCR (dPCR) technology to quantify cfDNA and cfRNA from human blood plasma in order to compare …
So far, no study has systematically compared the performance of cfDNA and cfRNA co-purification to increase sensitivity. RESULTS: First, we …
On determining the power of digital PCR experiments.
Vynck M, Vandesompele J, Thas O. Vynck M, et al. Among authors: vandesompele j, thas o. Anal Bioanal Chem. 2018 Sep;410(23):5731-5739. doi: 10.1007/s00216-018-1212-6. Epub 2018 Jun 30. Anal Bioanal Chem. 2018. PMID: 29961092
The experimental design that will be carried out to evaluate a nucleic acid quantification hypothesis determines the cost and feasibility of digital polymerase chain reaction (digital PCR) studies. Experiment design involves the calculation of the number of t …
The experimental design that will be carried out to evaluate a nucleic acid quantification hypothesis determines the cost and feasibility of …
The Future of Digital Polymerase Chain Reaction in Virology.
Vynck M, Trypsteen W, Thas O, Vandekerckhove L, De Spiegelaere W. Vynck M, et al. Among authors: trypsteen w, thas o, de spiegelaere w. Mol Diagn Ther. 2016 Oct;20(5):437-47. doi: 10.1007/s40291-016-0224-1. Mol Diagn Ther. 2016. PMID: 27351921 Review.
Driven by its potential benefits over currently available methods, and the recent development of commercial platforms, digital polymerase chain reaction (dPCR) has received increasing attention in virology research and diagnostics as a tool for the quantification of nuclei …
Driven by its potential benefits over currently available methods, and the recent development of commercial platforms, digital polyme …
31 results