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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2004 6
2005 9
2006 15
2007 16
2008 25
2009 23
2010 50
2011 49
2012 46
2013 82
2014 309
2015 350
2016 476
2017 428
2018 445
2019 488
2020 551
2021 526
2022 460
2023 532
2024 210

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4,655 results

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Page 1
Multiplex genome engineering using CRISPR/Cas systems.
Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Cong L, et al. Science. 2013 Feb 15;339(6121):819-23. doi: 10.1126/science.1231143. Epub 2013 Jan 3. Science. 2013. PMID: 23287718 Free PMC article.
We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells. Cas9 can also be converted into a nicking enzyme to facilitate homology- …
We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise c …
Spatially resolved single-cell translatomics at molecular resolution.
Zeng H, Huang J, Ren J, Wang CK, Tang Z, Zhou H, Zhou Y, Shi H, Aditham A, Sui X, Chen H, Lo JA, Wang X. Zeng H, et al. Science. 2023 Jun 30;380(6652):eadd3067. doi: 10.1126/science.add3067. Epub 2023 Jun 30. Science. 2023. PMID: 37384709
RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional gene modules. We mapped 5413 genes in mouse brain tissues, yielding spatially resolved single-cell translatomic profiles for 119,173 c …
RIBOmap profiling of 981 genes in HeLa cells revealed cell cycle-dependent translational control and colocalized translation of functional g …
Phage-assisted evolution and protein engineering yield compact, efficient prime editors.
Doman JL, Pandey S, Neugebauer ME, An M, Davis JR, Randolph PB, McElroy A, Gao XD, Raguram A, Richter MF, Everette KA, Banskota S, Tian K, Tao YA, Tolar J, Osborn MJ, Liu DR. Doman JL, et al. Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039. Cell. 2023. PMID: 37657419 Free PMC article.
These resulting editors (PE6a-g) enhance therapeutically relevant editing in patient-derived fibroblasts and primary human T-cells. PE6 variants also enable longer insertions to be installed in vivo following dual-AAV delivery, achieving 40% loxP insertion in the co …
These resulting editors (PE6a-g) enhance therapeutically relevant editing in patient-derived fibroblasts and primary human T-cells. PE6 vari …
Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species.
Tabebordbar M, Lagerborg KA, Stanton A, King EM, Ye S, Tellez L, Krunnfusz A, Tavakoli S, Widrick JJ, Messemer KA, Troiano EC, Moghadaszadeh B, Peacker BL, Leacock KA, Horwitz N, Beggs AH, Wagers AJ, Sabeti PC. Tabebordbar M, et al. Cell. 2021 Sep 16;184(19):4919-4938.e22. doi: 10.1016/j.cell.2021.08.028. Epub 2021 Sep 9. Cell. 2021. PMID: 34506722 Free PMC article.
Using this method, we identify a class of RGD motif-containing capsids that transduces muscle with superior efficiency and selectivity after intravenous injection in mice and non-human primates. We demonstrate substantially enhanced potency and therapeutic efficacy …
Using this method, we identify a class of RGD motif-containing capsids that transduces muscle with superior efficiency and selectivity after …
Distinct longevity mechanisms across and within species and their association with aging.
Tyshkovskiy A, Ma S, Shindyapina AV, Tikhonov S, Lee SG, Bozaykut P, Castro JP, Seluanov A, Schork NJ, Gorbunova V, Dmitriev SE, Miller RA, Gladyshev VN. Tyshkovskiy A, et al. Cell. 2023 Jun 22;186(13):2929-2949.e20. doi: 10.1016/j.cell.2023.05.002. Epub 2023 Jun 3. Cell. 2023. PMID: 37269831
The identified biomarkers revealed longevity interventions, including KU0063794, which extended mouse lifespan and healthspan. Overall, this study uncovers universal and distinct strategies of lifespan regulation within and across species and provides tools for discovering …
The identified biomarkers revealed longevity interventions, including KU0063794, which extended mouse lifespan and healthspan. Overal …
Mitochondrial metabolism promotes adaptation to proteotoxic stress.
Tsvetkov P, Detappe A, Cai K, Keys HR, Brune Z, Ying W, Thiru P, Reidy M, Kugener G, Rossen J, Kocak M, Kory N, Tsherniak A, Santagata S, Whitesell L, Ghobrial IM, Markley JL, Lindquist S, Golub TR. Tsvetkov P, et al. Nat Chem Biol. 2019 Jul;15(7):681-689. doi: 10.1038/s41589-019-0291-9. Epub 2019 May 27. Nat Chem Biol. 2019. PMID: 31133756 Free PMC article.
The mechanisms by which cells adapt to proteotoxic stress are largely unknown, but are key to understanding how tumor cells, particularly in vivo, are largely resistant to proteasome inhibitors. Analysis of cancer cell lines, mouse xenografts and patient-deri …
The mechanisms by which cells adapt to proteotoxic stress are largely unknown, but are key to understanding how tumor cells, particularly …
Modeling epigenetic lesions that cause gliomas.
Rahme GJ, Javed NM, Puorro KL, Xin S, Hovestadt V, Johnstone SE, Bernstein BE. Rahme GJ, et al. Cell. 2023 Aug 17;186(17):3674-3685.e14. doi: 10.1016/j.cell.2023.06.022. Epub 2023 Jul 25. Cell. 2023. PMID: 37494934
Coordinate inactivation of the Pdgfra insulator and Cdkn2a drives gliomagenesis in vivo. Despite locus synteny, the insulator is CpG-rich only in humans, a feature that may confer human glioma risk but complicates mouse modeling. Our study demonstrates the ca …
Coordinate inactivation of the Pdgfra insulator and Cdkn2a drives gliomagenesis in vivo. Despite locus synteny, the insulator …
A metastasis map of human cancer cell lines.
Jin X, Demere Z, Nair K, Ali A, Ferraro GB, Natoli T, Deik A, Petronio L, Tang AA, Zhu C, Wang L, Rosenberg D, Mangena V, Roth J, Chung K, Jain RK, Clish CB, Vander Heiden MG, Golub TR. Jin X, et al. Nature. 2020 Dec;588(7837):331-336. doi: 10.1038/s41586-020-2969-2. Epub 2020 Dec 9. Nature. 2020. PMID: 33299191 Free PMC article.
Most deaths from cancer are explained by metastasis, and yet large-scale metastasis research has been impractical owing to the complexity of in vivo models. Here we introduce an in vivo barcoding strategy that is capable of determining the metastatic p …
Most deaths from cancer are explained by metastasis, and yet large-scale metastasis research has been impractical owing to the complexity of …
Comparative transcriptomics reveals human-specific cortical features.
Jorstad NL, Song JHT, Exposito-Alonso D, Suresh H, Castro-Pacheco N, Krienen FM, Yanny AM, Close J, Gelfand E, Long B, Seeman SC, Travaglini KJ, Basu S, Beaudin M, Bertagnolli D, Crow M, Ding SL, Eggermont J, Glandon A, Goldy J, Kiick K, Kroes T, McMillen D, Pham T, Rimorin C, Siletti K, Somasundaram S, Tieu M, Torkelson A, Feng G, Hopkins WD, Höllt T, Keene CD, Linnarsson S, McCarroll SA, Lelieveldt BP, Sherwood CC, Smith K, Walsh CA, Dobin A, Gillis J, Lein ES, Hodge RD, Bakken TE. Jorstad NL, et al. Science. 2023 Oct 13;382(6667):eade9516. doi: 10.1126/science.ade9516. Epub 2023 Oct 13. Science. 2023. PMID: 37824638 Free PMC article.
The cognitive abilities of humans are distinctive among primates, but their molecular and cellular substrates are poorly understood. We used comparative single-nucleus transcriptomics to analyze samples of the middle temporal gyrus (MTG) from adult humans, chimpanzees, gor …
The cognitive abilities of humans are distinctive among primates, but their molecular and cellular substrates are poorly understood. …
Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy.
Wei J, Long L, Zheng W, Dhungana Y, Lim SA, Guy C, Wang Y, Wang YD, Qian C, Xu B, Kc A, Saravia J, Huang H, Yu J, Doench JG, Geiger TL, Chi H. Wei J, et al. Nature. 2019 Dec;576(7787):471-476. doi: 10.1038/s41586-019-1821-z. Epub 2019 Dec 11. Nature. 2019. PMID: 31827283 Free PMC article.
Adoptive cell therapy represents a new paradigm in cancer immunotherapy, but it can be limited by the poor persistence and function of transferred T cells(1). Here we use an in vivo pooled CRISPR-Cas9 mutagenesis screening approach to demonstrate that, by targeting …
Adoptive cell therapy represents a new paradigm in cancer immunotherapy, but it can be limited by the poor persistence and function of trans …
4,655 results