Rational Design of the Spatial Effect in a Fe(II)/α-Ketoglutarate-Dependent Dioxygenase Reverses the Regioselectivity of C(sp3)-H Bond Hydroxylation in Aliphatic Amino Acids.
Zheng C, Wei W, Wen J, Song W, Wu J, Wang R, Yin D, Chen X, Gao C, Liu J, Liu L.
Zheng C, et al.
Angew Chem Int Ed Engl. 2024 Aug 5;63(32):e202406060. doi: 10.1002/anie.202406060. Epub 2024 Jul 3.
Angew Chem Int Ed Engl. 2024.
PMID: 38789390
In this study, the Fe(II)/alpha-ketoglutarate-dependent dioxygenase from Bacillus subtilis (BlAH) was mined and found to catalyze hydroxylation at the gamma and delta sites of aliphatic amino acids. Crystal structure analysis, molecular dynamics simulations, and quantum ch …
In this study, the Fe(II)/alpha-ketoglutarate-dependent dioxygenase from Bacillus subtilis (BlAH) was mined and found to catalyze hyd …