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Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM.
Alahuhta M, Casteleijn MG, Neubauer P, Wierenga RK. Alahuhta M, et al. Among authors: casteleijn mg. Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):178-88. doi: 10.1107/S0907444907059021. Epub 2008 Jan 16. Acta Crystallogr D Biol Crystallogr. 2008. PMID: 18219118
Structure-based protein engineering efforts with a monomeric TIM variant: the importance of a single point mutation for generating an active site with suitable binding properties.
Alahuhta M, Salin M, Casteleijn MG, Kemmer C, El-Sayed I, Augustyns K, Neubauer P, Wierenga RK. Alahuhta M, et al. Among authors: casteleijn mg. Protein Eng Des Sel. 2008 Apr;21(4):257-66. doi: 10.1093/protein/gzn002. Epub 2008 Jan 31. Protein Eng Des Sel. 2008. PMID: 18239072
Characterization, Stability, and in Vivo Efficacy Studies of Recombinant Human CNTF and Its Permeation into the Neural Retina in ex Vivo Organotypic Retinal Explant Culture Models.
Itkonen J, Annala A, Tavakoli S, Arango-Gonzalez B, Ueffing M, Toropainen E, Ruponen M, Casteleijn MG, Urtti A. Itkonen J, et al. Among authors: casteleijn mg. Pharmaceutics. 2020 Jun 30;12(7):611. doi: 10.3390/pharmaceutics12070611. Pharmaceutics. 2020. PMID: 32629980 Free PMC article.
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